A statistical model for activation of Factor C by binding to LPS aggregates.
Eur Biophys J
; 48(8): 743-747, 2019 Dec.
Article
em En
| MEDLINE
| ID: mdl-31630219
ABSTRACT
Published data on Factor C activity at various LPS and Lipid A concentrations (Nakamura et al. in Eur J Biochem 17689, 1988; Kobayashi et al. in J Biol Chem 3725987, 2014) were rearranged to show that Factor C exhibited its maximum activity at a specific concentration of LPS. A statistical model was proposed for examining whether a single LPS molecule binding activates Factor C (monomeric activation) or dimerization of Factor C is necessary for the activation (dimeric activation). In the monomeric activation model the plots of the relative activity of Factor C against the molar ratio of LPS to Factor C were different from those in the published data. The plots in the dimeric activation model lie on a bell-shaped curve, whatever the Factor C concentration, matching the published data and indicating the appropriateness of that model. We suggest that Factor C is activated by multiple molecular interactions of Factor C with LPS aggregates on which it dimerises and that this explains why larger aggregates are less effective at activating Factor C than smaller ones.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Serina Endopeptidases
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Modelos Moleculares
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Lipopolissacarídeos
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Precursores Enzimáticos
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Proteínas de Artrópodes
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Eur Biophys J
Assunto da revista:
BIOFISICA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Japão