Intronic heterochromatin prevents cryptic transcription initiation in Arabidopsis.
Plant J
; 101(5): 1185-1197, 2020 03.
Article
em En
| MEDLINE
| ID: mdl-31647592
ABSTRACT
Intronic transposable elements (TEs) comprise a large proportion in eukaryotic genomes, but how they regulate the host genes remains to be explored. Our forward genetic screen disclosed the plant-specific RNA polymerases IV and V in suppressing intronic TE-mediated cryptic transcription initiation of a chimeric transcripts at FLC (FLCTE ). Initiation of FLCTE transcription is blocked by the locally formed intronic heterochromatin, which is directly associated with RNA Pol V to inhibit the entry of RNA Pol II and the occupancy of H3K4 methylation. Genome-wide Pol II Ser5p native elongation transcription sequencing revealed that a significant number of intronic heterochromatin-containing genes undergo this mechanism. This study sheds light on deeply understanding the function of intronic heterochromatin on host genes expression in eukaryotic genome.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Polimerases Dirigidas por DNA
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Elementos de DNA Transponíveis
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Heterocromatina
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Íntrons
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Arabidopsis
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Genoma de Planta
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Proteínas de Domínio MADS
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Proteínas de Arabidopsis
Idioma:
En
Revista:
Plant J
Assunto da revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China