Liver microsystems in vitro for drug response.
J Biomed Sci
; 26(1): 88, 2019 Oct 28.
Article
em En
| MEDLINE
| ID: mdl-31660980
ABSTRACT
Engineering approaches were adopted for liver microsystems to recapitulate cell arrangements and culture microenvironments in vivo for sensitive, high-throughput and biomimetic drug screening. This review introduces liver microsystems in vitro for drug hepatotoxicity, drug-drug interactions, metabolic function and enzyme induction, based on cell micropatterning, hydrogel biofabrication and microfluidic perfusion. The engineered microsystems provide varied microenvironments for cell culture that feature cell coculture with non-parenchymal cells, in a heterogeneous extracellular matrix and under controllable perfusion. The engineering methods described include cell micropatterning with soft lithography and dielectrophoresis, hydrogel biofabrication with photolithography, micromolding and 3D bioprinting, and microfluidic perfusion with endothelial-like structures and gradient generators. We discuss the major challenges and trends of liver microsystems to study drug response in vitro.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Preparações Farmacêuticas
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Avaliação Pré-Clínica de Medicamentos
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Microtecnologia
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Engenharia Celular
Limite:
Humans
Idioma:
En
Revista:
J Biomed Sci
Assunto da revista:
MEDICINA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Taiwan