Optimal detection of extended-spectrum ß-lactamase producers, carbapenemase producers, polymyxin-resistant Enterobacterales, and vancomycin-resistant enterococci from stools.
Diagn Microbiol Infect Dis
; 96(1): 114919, 2020 Jan.
Article
em En
| MEDLINE
| ID: mdl-31679814
ABSTRACT
This study compared the impact of different approaches, namely, nonenrichment, nonselective enrichment, and selective (antibiotic-containing) enrichment steps, for detecting extended-spectrum ß-lactamase producing Enterobacterales (ESBL-E), carbapenemase-producing Enterobacterales (CPE), polymyxin-resistant Enterobacterales (PMR-E), and vancomycin-resistant enterococci (VRE) from spiked stools. The use of a nonselective 18-h enrichment broth culture significantly improved the recovery rate of all types of resistant bacteria after their plating onto selective media. In addition, the detection of ESBL-E, CPE, PMR-E, and VRE was further improved when using an enrichment step using antibiotic-supplemented broths respectively supplemented with cefotaxime (0.1⯵g/mL), ertapenem (0.1⯵g/mL), colistin (0.5⯵g/mL), and vancomycin (1⯵g/mL). Therefore, we showed here that a screening strategy based on a selective broth enrichment step significantly contributes to an increased rate of detection of multidrug-resistant bacteria, which may be crucial in term of improvement of infection control.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Polimixinas
/
Farmacorresistência Bacteriana Múltipla
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Enterobacteriaceae
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Enterococos Resistentes à Vancomicina
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Suíça