Contemporary Tools for Regulating Gene Expression in Bacteria.
Trends Biotechnol
; 38(3): 316-333, 2020 03.
Article
em En
| MEDLINE
| ID: mdl-31679824
ABSTRACT
Insights from novel mechanistic paradigms in gene expression control have led to the development of new gene expression systems for bioproduction, control, and sensing applications. Coupled with a greater understanding of synthetic burden and modern creative biodesign approaches, contemporary bacterial gene expression tools and systems are emerging that permit fine-tuning of expression, enabling greater predictability and maximisation of specific productivity, while minimising deleterious effects upon cell viability. These advances have been achieved by using a plethora of regulatory tools, operating at all levels of the so-called 'central dogma' of molecular biology. In this review, we discuss these gene regulation tools in the context of their design, prototyping, integration into expression systems, and biotechnological application.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Engenharia Genética
/
Regulação Bacteriana da Expressão Gênica
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Trends Biotechnol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Reino Unido