Can miRNAs Serve as Potential Markers in Thermal Burn Injury: An In Silico Approach.

ABSTRACT

Burn injury has been a major cause of morbidity at global levels. They can occur by multiple agents, such as thermal radiation and chemicals. Among all, thermal burn is predominant and may require specialized treatment in some patients. Although various biomarkers are reportedly used in thermal burn for understanding the pathophysiology of the injury, their limitations prompt for the search of suitable markers that can address the depth and severity of the burn. MicroRNAs (miRNAs) are conserved noncoding molecules that seem to be the promising marker due to their role in multiple pathways and participation in different physiological processes of the body. The present review highlights the role of miRNAs in the repair of the wound and their interaction with specific genes in response to burn stress. Key miR candidates include miR-21, miR-29a, miR-378a-5p, miR-100, miR-27b, miR-200c, miR-150, miR-499-5p, miR-92a, miR-194, and miR-146b, which are identified for their respective targets involved in wound repair. Furthermore, bioinformatics and computational tools were used to confirm the miRNAs and their specific targets. Gene and miRNA expression data sets were downloaded from Research Collaboratory for Structural Bioinformatics Protein Data Bank Database and RNAComposer, respectively, and docked by PatchDock. The possible implications of the identified miRNAs could be in understanding the mechanism of burn injury. These can also be studied with the available drugs being used for burn injury. Apart from that, new intended molecules may also be tested for their effect on these miRNAs.