Gramella sabulilitoris sp. nov., isolated from a marine sand.
Int J Syst Evol Microbiol
; 70(2): 909-914, 2020 Feb.
Article
em En
| MEDLINE
| ID: mdl-31714202
ABSTRACT
A Gram-stain-negative, aerobic, non-spore-forming, motile by gliding and rod-shaped bacterial strain, designated HSMS-1T, was isolated from a marine sand collected from the Yellow Sea, Republic of Korea, and identified by a polyphasic taxonomic approach. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that HSMS-1T fell within the clade comprising the type strains of species of the genus Gramella. HSMS-1T exhibited 16S rRNA gene sequence similarity values of 99.0 and 98.7â% to the type strains of Gramella echinicola and Gramella sediminilitoris and of 93.3-98.5â% to the type strains of the other species of the genus Gramella. The ANI and dDDH values between HSMS-1T and the type strains of G. echinicola, Gramella gaetbulicola, Gramella forsetii, Gramella salexigens, Gramella portivictoriae and Gramella flava were 72.6-79.3â% and 17.4-22.2â%, respectively. Mean DNA-DNA relatedness value between HSMS-1T and the type strain of G. sediminilitoris was 18â%. HSMS-1T contained MK-6 as the predominant menaquinone and iso-C15ââ0, anteiso-C15ââ0, iso-C17ââ0 3-OH and iso-C16ââ0 as the major fatty acids. The major polar lipid of HSMS-1T was phosphatidylethanolamine. The DNA G+C content of HSMS-1T from genomic sequence data was 39.2â%. Distinguishing phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that HSMS-1T is separated from recognized species of the genus Gramella. On the basis of the data presented, strain HSMS-1T is considered to represent a novel species of the genus Gramella, for which the name Gramella sabulilitoris sp. nov. is proposed. The type strain is HSMS-1T(=KACC 19899T=NBRC 113648T).
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Flavobacteriaceae
/
Areia
País/Região como assunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article