The interaction of CaM7 and CNGC14 regulates root hair growth in Arabidopsis.
J Integr Plant Biol
; 62(7): 887-896, 2020 Jul.
Article
em En
| MEDLINE
| ID: mdl-31755194
ABSTRACT
Oscillations in cytosolic free calcium determine the polarity of tip-growing root hairs. The Ca2+ channel cyclic nucleotide gated channel 14 (CNGC14) contributes to the dynamic changes in Ca2+ concentration gradient at the root hair tip. However, the mechanisms that regulate CNGC14 are unknown. In this study, we detected a direct interaction between calmodulin 7 (CaM7) and CNGC14 through yeast two-hybrid and bimolecular fluorescence complementation assays. We demonstrated that the third EF-hand domain of CaM7 specifically interacts with the cytosolic C-terminal domain of CNGC14. A two-electrode voltage clamp assay showed that CaM7 completely inhibits CNGC14-mediated Ca2+ influx, suggesting that CaM7 negatively regulates CNGC14-mediated calcium signaling. Furthermore, CaM7 overexpressing lines phenocopy the short root hair phenotype of a cngc14 mutant and this phenotype is insensitive to changes in external Ca2+ concentrations. We, thus, identified CaM7-CNGC14 as a novel interacting module that regulates polar growth in root hairs by controlling the tip-focused Ca2+ signal.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Calmodulina
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Arabidopsis
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Raízes de Plantas
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Proteínas de Arabidopsis
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Canais de Cátion Regulados por Nucleotídeos Cíclicos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Integr Plant Biol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China