Localization microscopy at doubled precision with patterned illumination.

Cnossen, Jelmer; Hinsdale, Taylor; Thorsen, Rasmus Ø; Siemons, Marijn; Schueder, Florian; Jungmann, Ralf; Smith, Carlas S; Rieger, Bernd; Stallinga, Sjoerd

Cnossen, Jelmer; Hinsdale, Taylor; Thorsen, Rasmus Ø; Siemons, Marijn; Schueder, Florian; Jungmann, Ralf; Smith, Carlas S; Rieger, Bernd; Stallinga, Sjoerd.

Afiliação

Cnossen J; Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands.
Hinsdale T; Delft Center for Systems and Control, Delft University of Technology, Delft, the Netherlands.
Thorsen RØ; Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands.
Siemons M; Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands.
Schueder F; Department of Biology, University of Utrecht, Utrecht, the Netherlands.
Jungmann R; Department of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.
Smith CS; Max Planck Institute of Biochemistry, Martinsried, Germany.
Rieger B; Department of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.
Stallinga S; Max Planck Institute of Biochemistry, Martinsried, Germany.

Nat Methods ; 17(1): 59-63, 2020 01.

Article em En | MEDLINE | ID: mdl-31819263

ABSTRACT

ABSTRACT

MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

Assuntos

DNA/metabolismo; DNA/ultraestrutura; Iluminação/métodos; Microscopia de Fluorescência/métodos; Modelos Teóricos; Nanoestruturas/ultraestrutura; Imagem Individual de Molécula/métodos; Animais; Humanos; Iluminação/instrumentação; Nanotecnologia/métodos; Fótons

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Iluminação / DNA / Nanoestruturas / Imagem Individual de Molécula / Microscopia de Fluorescência / Modelos Teóricos Limite: Animals / Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Holanda

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