Localization microscopy at doubled precision with patterned illumination.
Nat Methods
; 17(1): 59-63, 2020 01.
Article
em En
| MEDLINE
| ID: mdl-31819263
ABSTRACT
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Iluminação
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DNA
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Nanoestruturas
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Imagem Individual de Molécula
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Microscopia de Fluorescência
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Modelos Teóricos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Holanda