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Genomic analysis of carbapenemase-producing Enterobacteriaceae in Queensland reveals widespread transmission of blaIMP-4 on an IncHI2 plasmid.
Roberts, Leah W; Catchpoole, Elizabeth; Jennison, Amy V; Bergh, Haakon; Hume, Anna; Heney, Claire; George, Narelle; Paterson, David L; Schembri, Mark A; Beatson, Scott A; Harris, Patrick N A.
Afiliação
  • Roberts LW; Australian Centre for Ecogenomics, The University of Queensland, Brisbane, QLD, Australia.
  • Catchpoole E; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia.
  • Jennison AV; Central Microbiology, Pathology Queensland, QLD, Australia.
  • Bergh H; Public Health Microbiology Laboratory, Queensland Health Forensic and Scientific Services, Queensland Department of Health, Coopers Plains, QLD, Australia.
  • Hume A; Central Microbiology, Pathology Queensland, QLD, Australia.
  • Heney C; Central Microbiology, Pathology Queensland, QLD, Australia.
  • George N; Central Microbiology, Pathology Queensland, QLD, Australia.
  • Paterson DL; Central Microbiology, Pathology Queensland, QLD, Australia.
  • Schembri MA; University of Queensland, Faculty of Medicine, UQ Centre for Clinical Research, Royal Brisbane & Women's Hospital, QLD, Australia.
  • Beatson SA; Australian Infectious Disease Research Centre, The University of Queensland, Brisbane, Australia.
  • Harris PNA; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia.
Microb Genom ; 6(1)2020 01.
Article em En | MEDLINE | ID: mdl-31860437
Carbapenemase-producing Enterobacteriaceae (CPE) are an increasingly common cause of healthcare-associated infections and may occasionally be identified in patients without extensive healthcare exposure. blaIMP-4 is the most frequently detected carbapenemase gene in Enterobacteriaceae within Australia, but little is known about the mechanisms behind its persistence. Here we used whole genome sequencing (WGS) to investigate the molecular epidemiology of blaIMP-4 in Queensland, Australia. In total, 107 CPE were collected between 2014 and 2017 and sent for WGS on an Illumina NextSeq500. Resistance genes and plasmid types were detected using a combination of read mapping and nucleotide comparison of de novo assemblies. Six isolates were additionally sequenced using Oxford Nanopore MinION to generate long-reads and fully characterize the context of the blaIMP-4 gene. Of 107 CPE, 93 carried the blaIMP-4 gene; 74/107 also carried an IncHI2 plasmid, suggesting carriage of the blaIMP-4 gene on an IncHI2 plasmid. Comparison of these isolates to a previously characterized IncHI2 plasmid pMS7884A (isolated from an Enterobacter hormaechei strain in Brisbane) suggested that all isolates carried a similar plasmid. Five of six representative isolates sequenced using Nanopore long-read technology carried IncHI2 plasmids harbouring the blaIMP-4 gene. While the vast majority of isolates represented E. hormaechei, several other species were also found to carry the IncHI2 plasmid, including Klebsiella species, Escherichia coli and Citrobacter species. Several clonal groups of E. hormaechei were also identified, suggesting that persistence of blaIMP-4 is driven by both presence on a common plasmid and clonal spread of certain E. hormaechei lineages.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Beta-Lactamases / Enterobacteriaceae / Infecções por Enterobacteriaceae Limite: Female / Humans / Male País/Região como assunto: Oceania Idioma: En Revista: Microb Genom Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Beta-Lactamases / Enterobacteriaceae / Infecções por Enterobacteriaceae Limite: Female / Humans / Male País/Região como assunto: Oceania Idioma: En Revista: Microb Genom Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Austrália