Strategies for Engineering and Rewiring Kinase Regulation.
Trends Biochem Sci
; 45(3): 259-271, 2020 03.
Article
em En
| MEDLINE
| ID: mdl-31866305
ABSTRACT
Eukaryotic protein kinases (EPKs) catalyze the transfer of a phosphate group onto another protein in response to appropriate regulatory cues. In doing so, they provide a primary means for cellular information transfer. Consequently, EPKs play crucial roles in cell differentiation and cell-cycle progression, and kinase dysregulation is associated with numerous disease phenotypes including cancer. Nonnative cues for synthetically regulating kinases are thus much sought after, both for dissecting cell signaling pathways and for pharmaceutical development. In recent years advances in protein engineering and sequence analysis have led to new approaches for manipulating kinase activity, localization, and in some instances specificity. These tools have revealed fundamental principles of intracellular signaling and suggest paths forward for the design of therapeutic allosteric kinase regulators.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Quinases
/
Engenharia de Proteínas
/
Neoplasias
Limite:
Humans
Idioma:
En
Revista:
Trends Biochem Sci
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos