Precise correlative method of Cryo-SXT and Cryo-FM for organelle identification.
J Synchrotron Radiat
; 27(Pt 1): 176-184, 2020 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-31868750
ABSTRACT
Cryogenic soft X-ray tomography (Cryo-SXT) is ideally suitable to image the 3D sub-cellular architecture and organization of cells with high resolution in the near-native preservation state. Cryogenic fluorescence microscopy (Cryo-FM) can determine the location of a molecule of interest that has been labeled with a fluorescent tag, thus revealing the function of the cells. To understand the relations between the sub-cellular architecture and the function of cells, correlative Cryo-SXT and Cryo-FM was applied. This method required the matching of images of different modalities, and the accuracy of the matching is important. Here, a precise correlative method of Cryo-SXT and Cryo-FM is introduced. The capability of matching images of different modalities with high resolution was verified by simulations and practical experiments, and the method was used to identify vacuoles and mitochondria.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Organelas
/
Microscopia Confocal
/
Imageamento Tridimensional
/
Microtomografia por Raio-X
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Microscopia de Fluorescência
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Synchrotron Radiat
Assunto da revista:
RADIOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article