Inactivation of three emerging viruses - severe acute respiratory syndrome coronavirus, Crimean-Congo haemorrhagic fever virus and Nipah virus - in platelet concentrates by ultraviolet C light and in plasma by methylene blue plus visible light.
Vox Sang
; 115(3): 146-151, 2020 Apr.
Article
em En
| MEDLINE
| ID: mdl-31930543
ABSTRACT
BACKGROUND:
Emerging viruses like severe acute respiratory syndrome coronavirus (SARS-CoV), Crimean-Congo haemorrhagic fever virus (CCHFV) and Nipah virus (NiV) have been identified to pose a potential threat to transfusion safety. In this study, the ability of the THERAFLEX UV-Platelets and THERAFLEX MB-Plasma pathogen inactivation systems to inactivate these viruses in platelet concentrates and plasma, respectively, was investigated. MATERIALS ANDMETHODS:
Blood products were spiked with SARS-CoV, CCHFV or NiV, and then treated with increasing doses of UVC light (THERAFLEX UV-Platelets) or with methylene blue (MB) plus increasing doses of visible light (MB/light; THERAFLEX MB-Plasma). Samples were taken before and after treatment with each illumination dose and tested for residual infectivity.RESULTS:
Treatment with half to three-fourths of the full UVC dose (0·2 J/cm2 ) reduced the infectivity of SARS-CoV (≥3·4 log), CCHFV (≥2·2 log) and NiV (≥4·3 log) to the limit of detection (LOD) in platelet concentrates, and treatment with MB and a fourth of the full light dose (120 J/cm2 ) decreased that of SARS-CoV (≥3·1 log), CCHFV (≥3·2 log) and NiV (≥2·7 log) to the LOD in plasma.CONCLUSION:
Our study demonstrates that both THERAFLEX UV-Platelets (UVC) and THERAFLEX MB-Plasma (MB/light) effectively reduce the infectivity of SARS-CoV, CCHFV and NiV in platelet concentrates and plasma, respectively.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Raios Ultravioleta
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Vírus da Febre Hemorrágica da Crimeia-Congo
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Inativação de Vírus
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Coronavírus Relacionado à Síndrome Respiratória Aguda Grave
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Vírus Nipah
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Luz
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Azul de Metileno
Limite:
Humans
Idioma:
En
Revista:
Vox Sang
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Alemanha