Combined antisclerostin antibody and parathyroid hormone (1-34) synergistically enhance the healing of bone defects in ovariectomized rats.
Z Gerontol Geriatr
; 53(2): 163-170, 2020 Mar.
Article
em En
| MEDLINE
| ID: mdl-31950363
ABSTRACT
Osteoporotic bones heal more slowly and ineffectively than normal bones. A combination of antibodies against sclerosing protein (Scl-Ab), and parathyroid hormone 1-34 (PTH 1-34) may improve healing. A standard osteoporotic rat model was established 12 weeks after bilateral ovarian resection (OVX). Bone defects were created in the right femora of 80 rats, which were randomly divided into 4 groups control, Scl-Ab (25â¯mg/kg twice weekly), PTH (60⯵g/kg of PTH 1-34 three times a week) and PTH plus Scl-Ab. After 12 weeks of treatment the rats were sacrificed and blood and the distal femora were harvested for biochemical evaluation, histology, microcomputed tomography and biomechanical testing. Compared to the control group, monotherapy and combination therapy with PTH and/or Scl-Ab promoted the formation of new bone, enhanced maximum femoral loading and increased the levels of procollagen type I Nterminal propeptide (PINP) and osteocalcin. The administration of PTHâ¯+ Scl-Ab maximally enhanced bone defect healing. Combination treatment was better than either treatment alone, indicating a synergistic effect.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hormônio Paratireóideo
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Remodelação Óssea
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Consolidação da Fratura
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Proteínas Morfogenéticas Ósseas
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Anticorpos
Tipo de estudo:
Clinical_trials
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Z Gerontol Geriatr
Assunto da revista:
GERIATRIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China