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Evolutionary Persistence of DNA Methylation for Millions of Years after Ancient Loss of a De Novo Methyltransferase.
Catania, Sandra; Dumesic, Phillip A; Pimentel, Harold; Nasif, Ammar; Stoddard, Caitlin I; Burke, Jordan E; Diedrich, Jolene K; Cook, Sophie; Shea, Terrance; Geinger, Elizabeth; Lintner, Robert; Yates, John R; Hajkova, Petra; Narlikar, Geeta J; Cuomo, Christina A; Pritchard, Jonathan K; Madhani, Hiten D.
Afiliação
  • Catania S; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Dumesic PA; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Pimentel H; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Nasif A; MRC London Institute of Medical Sciences (LMS), Reprogramming and Chromatin Group, Du Cane Road, W12 0NN London, UK; Institute of Clinical Sciences, Imperial College Faculty of Medicine, Du Cane Rd, W12 0NN London, UK.
  • Stoddard CI; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Burke JE; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Diedrich JK; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Cook S; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Shea T; Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Geinger E; Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Lintner R; Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Yates JR; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Hajkova P; MRC London Institute of Medical Sciences (LMS), Reprogramming and Chromatin Group, Du Cane Road, W12 0NN London, UK; Institute of Clinical Sciences, Imperial College Faculty of Medicine, Du Cane Rd, W12 0NN London, UK.
  • Narlikar GJ; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Cuomo CA; Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Pritchard JK; Department of Biology, Stanford University, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Madhani HD; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA; Chan-Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address: hitenmadhani@gmail.com.
Cell ; 180(2): 263-277.e20, 2020 01 23.
Article em En | MEDLINE | ID: mdl-31955845
Cytosine methylation of DNA is a widespread modification of DNA that plays numerous critical roles. In the yeast Cryptococcus neoformans, CG methylation occurs in transposon-rich repeats and requires the DNA methyltransferase Dnmt5. We show that Dnmt5 displays exquisite maintenance-type specificity in vitro and in vivo and utilizes similar in vivo cofactors as the metazoan maintenance methylase Dnmt1. Remarkably, phylogenetic and functional analysis revealed that the ancestral species lost the gene for a de novo methylase, DnmtX, between 50-150 mya. We examined how methylation has persisted since the ancient loss of DnmtX. Experimental and comparative studies reveal efficient replication of methylation patterns in C. neoformans, rare stochastic methylation loss and gain events, and the action of natural selection. We propose that an epigenome has been propagated for >50 million years through a process analogous to Darwinian evolution of the genome.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Metilação de DNA / Cryptococcus neoformans / Metiltransferases Idioma: En Revista: Cell Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Metilação de DNA / Cryptococcus neoformans / Metiltransferases Idioma: En Revista: Cell Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos