Methods for macrophage differentiation and in vitro generation of human tumor associated-like macrophages.
Methods Enzymol
; 632: 113-131, 2020.
Article
em En
| MEDLINE
| ID: mdl-32000892
ABSTRACT
Tumor-associated macrophages (TAMs) are becoming a promising target for cancer immunotherapy. Significant efforts have been made to study the detrimental role of TAMs both in vivo and in vitro. However, it remains challenging to isolate these macrophages to study their function in human cancers and there is the need to seek alternatives to address these limitations. In this review, we will focus on the three most relevant approaches to obtain in vitro fully differentiated macrophages i.e. peripheral blood, immortalized cell lines such as THP-1 or human induced pluripotent stem cells. We will also provide protocols for the polarization of human macrophages to a TAM-like cells in vitro.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Macrófagos Associados a Tumor
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Reino Unido