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Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo.
Wu, Jianglai; Liang, Yajie; Chen, Shuo; Hsu, Ching-Lung; Chavarha, Mariya; Evans, Stephen W; Shi, Dongqing; Lin, Michael Z; Tsia, Kevin K; Ji, Na.
Afiliação
  • Wu J; Department of Physics, University of California, Berkeley, CA, USA.
  • Liang Y; Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong, China.
  • Chen S; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Hsu CL; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Chavarha M; Department of Physics, University of California, Berkeley, CA, USA.
  • Evans SW; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Shi D; Department of Bioengineering, Stanford University, Stanford, CA, USA.
  • Lin MZ; Department of Bioengineering, Stanford University, Stanford, CA, USA.
  • Tsia KK; Department of Bioengineering, Stanford University, Stanford, CA, USA.
  • Ji N; Department of Bioengineering, Stanford University, Stanford, CA, USA.
Nat Methods ; 17(3): 287-290, 2020 03.
Article em En | MEDLINE | ID: mdl-32123392
Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 µm below the brain surface in head-fixed awake mice.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Encéfalo / Fótons / Microscopia de Fluorescência por Excitação Multifotônica / Neurônios Limite: Animals Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
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Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Encéfalo / Fótons / Microscopia de Fluorescência por Excitação Multifotônica / Neurônios Limite: Animals Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos