Isoenzyme studies on Toxoplasma gondii isolates using isoelectric focusing.
Zentralbl Bakteriol Mikrobiol Hyg A
; 268(4): 476-81, 1988 Jun.
Article
em En
| MEDLINE
| ID: mdl-3213318
ABSTRACT
Zymogram analysis using isoelectric focusing on polyacrylamide gels was performed to characterize and distinguish two Toxoplasma gondii isolates ("strains" BK and RH). The activity of the following 14 enzymes in the cell lysates was investigated IDH, MDH, ME, 6PG, G6P, LDH, IPO, HEX, PGM, EST, ALP, ACP, LAP, and PGI. Nine enzymes (IDH, G6P, LDH, HEX, PGM, EST, ALP, ACP, and PGI) showed distinct and reproducible banding patterns, and four of them (IDH, G6P, EST, PGI) enabled a reliable distinction of the two Toxoplasma gondii isolates. A contamination of the parasite extracts with host proteins could be excluded by comparison of the enzyme activities of the Toxoplasma isolates with mouse peritoneal exudate cells. Isoenzyme analysis proved to be a helpful method for a characterization and a distinction of Toxoplasma gondii isolates.
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Base de dados:
MEDLINE
Assunto principal:
Toxoplasma
/
Isoenzimas
Limite:
Animals
Idioma:
En
Revista:
Zentralbl Bakteriol Mikrobiol Hyg A
Ano de publicação:
1988
Tipo de documento:
Article