Efficiently accumulating germ-like stem cells from mouse postnatal ovary by in situ tissue culture.
Dev Growth Differ
; 62(4): 223-231, 2020 May.
Article
em En
| MEDLINE
| ID: mdl-32189336
ABSTRACT
Although recent studies have revealed that germline stem cells (GSCs) exist in the mouse postnatal ovary, how to efficiently obtain GSCs for regenerating neo-oogenesis is still a technical challenge. Here, we report that using in situ tissue culture we can efficiently accumulate large amounts of proliferating germ-like cells from mouse postnatal ovaries. Usually, more than 10,000 germ-like cells can be derived from one ovary by this method, and over 20% of these cells can grow into germ-like cells with self-renewal, which thus can serve as a good cell pool to isolate GSCs by other cell assorting methods such as FACS. This method is simple and time-saving, which should be useful for in future studies on mouse GSCs.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ovário
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Células-Tronco
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Técnicas de Cultura de Tecidos
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Células Germinativas
Limite:
Animals
Idioma:
En
Revista:
Dev Growth Differ
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China