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Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts.
Grimmer, Julia; Helm, Stefan; Dobritzsch, Dirk; Hause, Gerd; Shema, Gerta; Zahedi, René P; Baginsky, Sacha.
Afiliação
  • Grimmer J; Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3a, 06120, Halle, Saale, Germany.
  • Helm S; Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3a, 06120, Halle, Saale, Germany.
  • Dobritzsch D; Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3a, 06120, Halle, Saale, Germany.
  • Hause G; Biocenter of the University, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle, Saale, Germany.
  • Shema G; Leibniz-Institut für Analytische Wissenschaften -ISAS- e.V., Bunsen-Kirchhoff-Straße 11, 44139, Dortmund, Germany.
  • Zahedi RP; Leibniz-Institut für Analytische Wissenschaften -ISAS- e.V., Bunsen-Kirchhoff-Straße 11, 44139, Dortmund, Germany.
  • Baginsky S; Segal Cancer Proteomics Centre, Lady Davis Institute, Jewish General Hospital, McGill University, 3755 Côte Ste-Catherine Road, Montreal, QC, H3T 1E2, Canada.
Nat Commun ; 11(1): 1662, 2020 04 03.
Article em En | MEDLINE | ID: mdl-32245955
The proteasome is an essential protein-degradation machinery in eukaryotic cells that controls protein turnover and thereby the biogenesis and function of cell organelles. Chloroplasts import thousands of nuclear-encoded precursor proteins from the cytosol, suggesting that the bulk of plastid proteins is transiently exposed to the cytosolic proteasome complex. Therefore, there is a cytosolic equilibrium between chloroplast precursor protein import and proteasomal degradation. We show here that a shift in this equilibrium, induced by mild genetic proteasome impairment, results in elevated precursor protein abundance in the cytosol and significantly increased accumulation of functional photosynthetic complexes in protein import-deficient chloroplasts. Importantly, a proteasome lid mutant shows improved photosynthetic performance, even in the absence of an import defect, signifying that functional precursors are continuously degraded. Hence, turnover of plastid precursors in the cytosol represents a mechanism to constrain thylakoid membrane assembly and photosynthetic electron transport.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cloroplastos / Arabidopsis / Proteínas de Arabidopsis / Complexo de Proteínas do Centro de Reação Fotossintética / Complexo de Endopeptidases do Proteassoma Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cloroplastos / Arabidopsis / Proteínas de Arabidopsis / Complexo de Proteínas do Centro de Reação Fotossintética / Complexo de Endopeptidases do Proteassoma Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Alemanha