Nitrogen Deficiency-Induced Decrease in Cytokinins Content Promotes Rice Seminal Root Growth by Promoting Root Meristem Cell Proliferation and Cell Elongation.
Cells
; 9(4)2020 04 09.
Article
em En
| MEDLINE
| ID: mdl-32283600
Rice (Oryza sativa L.) seedlings grown under nitrogen (N) deficiency conditions show a foraging response characterized by increased root length. However, the mechanism underlying this developmental plasticity is still poorly understood. In this study, the mechanism by which N deficiency influences rice seminal root growth was investigated. The results demonstrated that compared with the control (1 mM N) treatment, N deficiency treatments strongly promoted seminal root growth. However, the N deficiency-induced growth was negated by the application of zeatin, which is a type of cytokinin (CK). Moreover, the promotion of rice seminal root growth was correlated with a decrease in CK content, which was due to the N deficiency-mediated inhibition of CK biosynthesis through the down-regulation of CK biosynthesis genes and an enhancement of CK degradation through the up-regulation of CK degradation genes. In addition, the N deficiency-induced decrease in CK content not only enhanced the root meristem cell proliferation rate by increasing the meristem cell number via the down-regulation of OsIAA3 and up-regulation of root-expressed OsPLTs, but also promoted root cell elongation by up-regulating cell elongation-related genes, including root-specific OsXTHs and OsEXPs. Taken together, our data suggest that an N deficiency-induced decrease in CK content promotes the seminal root growth of rice seedlings by promoting root meristem cell proliferation and cell elongation.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Oryza
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Raízes de Plantas
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Regulação da Expressão Gênica de Plantas
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Citocininas
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Nitrogênio
Idioma:
En
Revista:
Cells
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China