Viral vectors for neuronal cell type-specific visualization and manipulations.
Curr Opin Neurobiol
; 63: 67-76, 2020 08.
Article
em En
| MEDLINE
| ID: mdl-32344323
ABSTRACT
Characterizing neuronal cell types demands efficient strategies for specific labeling and manipulation of individual subtypes to dissect their connectivity and functions. Recombinant viral technology offers a powerful toolbox for targeted transgene expression in specific neuronal populations. In order to achieve cell type-specific targeting, exciting progress has been made to alter viral tropisms, design rational delivery strategies, and drive selective expression patterns with engineered DNA sequences in viral genomes. For the latter case, emerging single-cell genomic analyses provide rich databases. In this review, we will summarize current status, and point out challenges, of using viral vectors for neuronal cell type-specific visualization and manipulations. With concerted efforts, progress will continue to be made toward developing viral vectors for the vast array of neuronal subtypes in the mammalian nervous system.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Transferência de Genes
/
Vetores Genéticos
Limite:
Animals
Idioma:
En
Revista:
Curr Opin Neurobiol
Assunto da revista:
BIOLOGIA
/
NEUROLOGIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos