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Relative lipid oxidation associates directly with mitochondrial fusion phenotype and mitochondria-sarcoplasmic reticulum interactions in human skeletal muscle.
Castro-Sepulveda, Mauricio; Jannas-Vela, Sebastian; Fernández-Verdejo, Rodrigo; Ávalos-Allele, Daniela; Tapia, German; Villagrán, Claudio; Quezada, Nicolas; Zbinden-Foncea, Hermann.
Afiliação
  • Castro-Sepulveda M; Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae, Santiago, Chile.
  • Jannas-Vela S; Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Fernández-Verdejo R; Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae, Santiago, Chile.
  • Ávalos-Allele D; Carrera de Nutrición y Dietética, Departamento de Ciencias de la Salud, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Tapia G; Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae, Santiago, Chile.
  • Villagrán C; Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae, Santiago, Chile.
  • Quezada N; Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae, Santiago, Chile.
  • Zbinden-Foncea H; Departamento de Cirugía Digestiva, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
Am J Physiol Endocrinol Metab ; 318(6): E848-E855, 2020 06 01.
Article em En | MEDLINE | ID: mdl-32369416
ABSTRACT
Disturbances in skeletal muscle lipid oxidation might induce ectopic fat deposition and lipotoxicity. Nevertheless, the cellular mechanisms that regulate skeletal muscle lipid oxidation have not been fully determined. We aimed to determine whether there was an association between relative whole body lipid oxidation and mitochondrial size or mitochondria-sarcoplasmic reticulum interactions in the skeletal muscle. Twelve healthy men were included [mean (standard deviation), 24.7 (1.5) yr old, 24.4 (2.6) kg/m2]. The respiratory quotient (RQ) was used to estimate relative lipid oxidation at rest and during exercise (50% maximal oxygen consumption, 600 kcal expended). A skeletal muscle biopsy was obtained from the vastus lateralis at rest. Transmission electron microscopy was used to determine mitochondrial size and mitochondria-sarcoplasmic reticulum interactions (≤50 nm of distance between organelles). Protein levels of fusion/fission regulators were measured in skeletal muscle by Western blot. Resting RQ and exercise RQ associated inversely with intermyofibrillar mitochondrial size (r = -0.66 and r = -0.60, respectively, P < 0.05). Resting RQ also associated inversely with the percentage of intermyofibrillar mitochondria-sarcoplasmic reticulum interactions (r = -0.62, P = 0.03). Finally, intermyofibrillar mitochondrial size associated inversely with lipid droplet density (r = -0.66, P = 0.01) but directly with mitochondria fusion-to-fission ratio (r = 0.61, P = 0.03). Our results show that whole body lipid oxidation is associated with skeletal muscle intermyofibrillar mitochondrial size, fusion phenotype, and mitochondria-sarcoplasmic-reticulum interactions in nondiabetic humans.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Retículo Sarcoplasmático / Exercício Físico / Fibras Musculares Esqueléticas / Músculo Quadríceps / Metabolismo dos Lipídeos / Dinâmica Mitocondrial / Mitocôndrias Tipo de estudo: Risk_factors_studies Limite: Adolescent / Adult / Humans / Male Idioma: En Revista: Am J Physiol Endocrinol Metab Assunto da revista: ENDOCRINOLOGIA / FISIOLOGIA / METABOLISMO Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Retículo Sarcoplasmático / Exercício Físico / Fibras Musculares Esqueléticas / Músculo Quadríceps / Metabolismo dos Lipídeos / Dinâmica Mitocondrial / Mitocôndrias Tipo de estudo: Risk_factors_studies Limite: Adolescent / Adult / Humans / Male Idioma: En Revista: Am J Physiol Endocrinol Metab Assunto da revista: ENDOCRINOLOGIA / FISIOLOGIA / METABOLISMO Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile