Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing.
Nat Commun
; 11(1): 2284, 2020 05 08.
Article
em En
| MEDLINE
| ID: mdl-32385250
ABSTRACT
Manipulation of proteins by chemical modification is a powerful way to decipher their function. However, most ribosome-dependent and semi-synthetic methods have limitations in the number and type of modifications that can be introduced, especially in live cells. Here, we present an approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into proteins expressed in eukaryotic cells. We insert synthetic peptides into GFP, NaV1.5 and P2X2 receptors via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating protein function. We anticipate the approach will overcome some drawbacks of existing protein enigineering methods.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Processamento de Proteína
/
Trans-Splicing
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Commun
Assunto da revista:
BIOLOGIA
/
CIENCIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Dinamarca