Biochemical characterization of a novel &#945;-L-fucosidase from Pedobacter sp. and its application in synthesis of 3'-fucosyllactose and 2'-fucosyllactose.

Shi, Ran; Ma, Junwen; Yan, Qiaojuan; Yang, Shaoqing; Fan, Zhihong; Jiang, Zhengqiang

Biochemical characterization of a novel α-L-fucosidase from Pedobacter sp. and its application in synthesis of 3'-fucosyllactose and 2'-fucosyllactose.

Shi, Ran; Ma, Junwen; Yan, Qiaojuan; Yang, Shaoqing; Fan, Zhihong; Jiang, Zhengqiang.

Afiliação

Shi R; Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing, 100083, China.
Ma J; Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing, 100083, China.
Yan Q; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing, 100083, China.
Yang S; Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing, 100083, China.
Fan Z; Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing, 100083, China.
Jiang Z; Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing, 100083, China. zhqjiang@cau.edu.cn.

Appl Microbiol Biotechnol ; 104(13): 5813-5826, 2020 Jul.

Article em En | MEDLINE | ID: mdl-32388762

Assuntos

Proteínas de Bactérias/metabolismo; Oligossacarídeos/biossíntese; Pedobacter/enzimologia; Trissacarídeos/biossíntese; alfa-L-Fucosidase/metabolismo; Sequência de Aminoácidos; Proteínas de Bactérias/química; Proteínas de Bactérias/genética; Proteínas de Bactérias/isolamento & purificação; Escherichia coli/genética; Escherichia coli/metabolismo; Glicosídeos/metabolismo; Glicosilação; Concentração de Íons de Hidrogênio; Hidrólise; Lactose/metabolismo; Peso Molecular; Pedobacter/genética; Prebióticos; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/isolamento & purificação; Proteínas Recombinantes/metabolismo; Especificidade por Substrato; Temperatura; alfa-L-Fucosidase/química; alfa-L-Fucosidase/genética; alfa-L-Fucosidase/isolamento & purificação

Palavras-chave

2'-fucosyllactose; 3'-fucosyllactose; Pedobacter sp.; Transglycosylation; α-L-fucosidase

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Proteínas de Bactérias / Trissacarídeos / Pedobacter / Alfa-L-Fucosidase Idioma: En Revista: Appl Microbiol Biotechnol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

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