A new sample applicator for isoelectric focusing in horizontal polyacrylamide gels.
Electrophoresis
; 9(9): 485-8, 1988 Sep.
Article
em En
| MEDLINE
| ID: mdl-3243246
ABSTRACT
A new sample applicator for horizontal flat gels is described. The applicator is practically safe against contamination from adjacent samples and can be used for all types of electrophoretic separations including a concentration step for either the sample (i.e. disc electrophoresis) or the separated zones (i.e. isoelectric focusing). The applicator is a piece of flat glass with 26 or 51 parallel 2 mm wide grooves, drilled at distances of 9 or 4.5 mm. Samples, maximally 25 or 50, are applied to the areas between the grooves. By inverting the applicator, the samples are brought into close vicinity to the gel surface and the pendant droplets expand by capillary attraction into the slits between the glass and gel with resultant even distribution across the lanes of 2.5 or 7 mm width. The applicator can be used for separations with and without protection of the electrophoretic setup by paraffin oil and allows for fast multiple handling of samples by means of appropriate syringes and microtiter plates.
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Base de dados:
MEDLINE
Assunto principal:
Globinas
/
Focalização Isoelétrica
Limite:
Humans
/
Newborn
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
1988
Tipo de documento:
Article