Dynamic Stabilization of DNA Assembly by Using Pyrrole-Imidazole Polyamide.
Chembiochem
; 21(20): 2912-2915, 2020 10 15.
Article
em En
| MEDLINE
| ID: mdl-32458592
ABSTRACT
We used N-methylpyrrole (Py)-N-methylimidazole-(Im) polyamide as an exogenous agent to modulate the formation of DNA assemblies at specific double-stranded sequences. The concept was demonstrated on the hybridization chain reaction that forms linear DNA. Through a series of melting curve analyses, we demonstrated that the binding of Py-Im polyamide positively influenced both the HCR initiation and elongation steps. In particular, Py-Im polyamide was found to drastically stabilize the DNA duplex such that its thermal stability approached that of an equivalent hairpin structure. Also, the polyamide served as an anchor between hairpin pairs in the HCR assembly, thus improving the originally weak interstrand stability. We hope that these proof-of-concept results can inspire future use of Py-Im polyamide as a molecular tool to modulate the formation of DNA assemblies.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pirróis
/
DNA
/
Imidazóis
/
Nylons
Idioma:
En
Revista:
Chembiochem
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Singapura