Crosstalk between androgen and Wnt/ß-catenin leads to changes of wool density in FGF5-knockout sheep.
Cell Death Dis
; 11(5): 407, 2020 05 29.
Article
em En
| MEDLINE
| ID: mdl-32472005
ABSTRACT
Fibroblast growth factor 5 (FGF5) is a famous dominant inhibitor of anagen phase of hair cycle. Mutations of FGF5 gene result in a longer wool in mice, donkeys, dogs, cats, and even in human eyelashes. Sheep is an important source of wool production. How to improve the production of wool quickly and effectively is an urgent problem to be solved. In this study, we generated five FGF5-knockout Dorper sheep by the CRISPR/Cas9 system. The expression level of FGF5 mRNA in knockout (KO) sheep decreased significantly, and all FGF5 proteins were dysfunctional. The KO sheep displayed a significant increase in fine-wool and active hair-follicle density. The crosstalk between androgen and Wnt/ß-catenin signaling downstream of FGF5 gene plays a key role. We established downstream signaling cascades for the first time, including FGF5, FGFR1, androgen, AR, Wnt/ß-catenin, Shh/Gli2, c-MYC, and KRTs. These findings further improved the function of FGF5 gene, and provided therapeutic ideas for androgen alopecia.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Lã
/
Ovinos
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Fator 5 de Crescimento de Fibroblastos
/
Proteínas Wnt
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Beta Catenina
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Técnicas de Inativação de Genes
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Androgênios
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Cell Death Dis
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China