Modulation of ERG Genes Expression in Clinical Isolates of Candida tropicalis Susceptible and Resistant to Fluconazole and Itraconazole.
Mycopathologia
; 185(4): 675-684, 2020 Aug.
Article
em En
| MEDLINE
| ID: mdl-32500312
ABSTRACT
Candida tropicalis is a non-albicans Candida specie that causes candidosis in several countries, including Brazil. However, little is known about the mechanisms of drug resistance in C. tropicalis infections. In this study, we used clinical isolates of C. tropicalis susceptible as well as resistant to either Fluconazole or Itraconazole to assess the relationship between drug resistance and the expression of ERG and efflux pump genes. Our results showed that the main mechanism of resistance against both Fluconazole and Itraconazole in this specie is through the up-regulation of ERG rather than that of the efflux pump genes. We demonstrated that, although pre-treatment with azole drugs increases the expression of both ERG6 and ERG11 genes, the resistant or susceptible dose-dependent (SDD) samples are able to maintain high expression levels of these genes for longer periods of time than the susceptible samples.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fluconazol
/
Itraconazol
/
Farmacorresistência Fúngica
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Candida tropicalis
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Genes Fúngicos
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Antifúngicos
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Mycopathologia
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil