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Cartography of opportunistic pathogens and antibiotic resistance genes in a tertiary hospital environment.
Chng, Kern Rei; Li, Chenhao; Bertrand, Denis; Ng, Amanda Hui Qi; Kwah, Junmei Samantha; Low, Hwee Meng; Tong, Chengxuan; Natrajan, Maanasa; Zhang, Michael Hongjie; Xu, Licheng; Ko, Karrie Kwan Ki; Ho, Eliza Xin Pei; Av-Shalom, Tamar V; Teo, Jeanette Woon Pei; Khor, Chiea Chuen; Chen, Swaine L; Mason, Christopher E; Ng, Oon Tek; Marimuthu, Kalisvar; Ang, Brenda; Nagarajan, Niranjan.
Afiliação
  • Chng KR; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Li C; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Bertrand D; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Ng AHQ; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Kwah JS; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Low HM; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Tong C; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Natrajan M; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Zhang MH; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Xu L; Information Systems Technology and Design, Singapore University of Technology and Design, Singapore, Singapore.
  • Ko KKK; Department of Microbiology, Singapore General Hospital, Singapore, Singapore.
  • Ho EXP; Department of Molecular Pathology, Singapore General Hospital, Singapore, Singapore.
  • Av-Shalom TV; Duke-NUS Graduate Medical School, Singapore, Singapore.
  • Teo JWP; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Khor CC; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Chen SL; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Ng OT; Computational and Systems Biology, Genome Institute of Singapore, Singapore, Singapore.
  • Marimuthu K; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.
  • Ang B; National Centre for Infectious Diseases, Singapore, Singapore.
  • Nagarajan N; Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore, Singapore.
Nat Med ; 26(6): 941-951, 2020 06.
Article em En | MEDLINE | ID: mdl-32514171
ABSTRACT
Although disinfection is key to infection control, the colonization patterns and resistomes of hospital-environment microbes remain underexplored. We report the first extensive genomic characterization of microbiomes, pathogens and antibiotic resistance cassettes in a tertiary-care hospital, from repeated sampling (up to 1.5 years apart) of 179 sites associated with 45 beds. Deep shotgun metagenomics unveiled distinct ecological niches of microbes and antibiotic resistance genes characterized by biofilm-forming and human-microbiome-influenced environments with corresponding patterns of spatiotemporal divergence. Quasi-metagenomics with nanopore sequencing provided thousands of high-contiguity genomes, phage and plasmid sequences (>60% novel), enabling characterization of resistome and mobilome diversity and dynamic architectures in hospital environments. Phylogenetics identified multidrug-resistant strains as being widely distributed and stably colonizing across sites. Comparisons with clinical isolates indicated that such microbes can persist in hospitals for extended periods (>8 years), to opportunistically infect patients. These findings highlight the importance of characterizing antibiotic resistance reservoirs in hospitals and establish the feasibility of systematic surveys to target resources for preventing infections.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecção Hospitalar / Controle de Infecções / Farmacorresistência Bacteriana / Equipamentos e Provisões Hospitalares / Microbiota Tipo de estudo: Prognostic_studies Limite: Humans País/Região como assunto: Asia Idioma: En Revista: Nat Med Assunto da revista: BIOLOGIA MOLECULAR / MEDICINA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Singapura

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecção Hospitalar / Controle de Infecções / Farmacorresistência Bacteriana / Equipamentos e Provisões Hospitalares / Microbiota Tipo de estudo: Prognostic_studies Limite: Humans País/Região como assunto: Asia Idioma: En Revista: Nat Med Assunto da revista: BIOLOGIA MOLECULAR / MEDICINA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Singapura