Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT.
Nat Methods
; 17(8): 789-791, 2020 08.
Article
em En
| MEDLINE
| ID: mdl-32601424
ABSTRACT
DNA-PAINT's imaging speed has recently been significantly enhanced by optimized sequence design and buffer conditions. However, this implementation has not reached an ultimate speed limit and is only applicable to imaging of single targets. To further improve acquisition speed, we introduce concatenated, periodic DNA sequence motifs, yielding up to 100-fold-faster sampling in comparison to traditional DNA-PAINT. We extend this approach to six orthogonal sequence motifs, now enabling speed-optimized multiplexed imaging.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Imagem Molecular
Limite:
Humans
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Alemanha