Lactobacillus paraplantarum THG-G10 as a potential anti-acne agent with anti-bacterial and anti-inflammatory activities.
Anaerobe
; 64: 102243, 2020 Aug.
Article
em En
| MEDLINE
| ID: mdl-32712375
ABSTRACT
Cutibacterium acnes (formerly Propionibacterium acnes) is the main bacterium targeted for the prevention and medical treatment of acne vulgaris. Lactic acid bacteria (LAB) are a group of microorganisms classified by their ability to produce lactic acid through fermentation. Although the activities of LAB have been studied, their potential anti-acne effects are not well known. Here, Lactobacillus paraplantarum THG-G10, which has anti-bacterial activity against C. acnes, was isolated from traditional Kimchi in Republic of Korea. The anti-acne effects of dried cell-free supernatant of L. paraplantarum THG-G10 (DC-G10) were evaluated by determining its anti-microbial and anti-inflammatory activities. Anti-microbial activity was examined by a broth dilution assay 25â¯mg/ml of DC-G10 inhibited the growth of C. acnes KCTC 5012 and KACC 1194; salicylic acid and benzoyl peroxide for acne treatment inhibited the growth of C. acnes KCTC 5012 and KACC 11946â¯at concentrations of 1.25 and 7.5â¯mg/ml, respectively; and tea tree oil inhibited the growth of C. acnes KCTC 5012 but not the growth of C. acnes KACC 11946â¯at 50â¯mg/ml. Anti-inflammatory activity was evaluated by a nitric oxide (NO) assay only DC-G10 and ascorbic acid reduced LPS-induced NO production in RAW 264.7â¯cells in a dose-dependent manner. In addition, the toxicities of erythromycin, salicylic acid, benzoyl peroxide, tea tree oil, and DC-G10 were examined in HaCaT cells and normal human dermal fibroblasts (NHDFs). In these cells, the cytotoxic effects of DC-G10 were weaker than the effects of erythromycin, benzoyl peroxide, and ascorbic acid. Furthermore, scanning electron microscopy revealed that DC-G10 induces deleterious morphological changes in the bacterial cell membrane. These results demonstrate that DC-G10 may be an effective and safe treatment for acne vulgaris.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Propionibacterium acnes
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Acne Vulgar
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Lactobacillus
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Anti-Inflamatórios
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Antibacterianos
Limite:
Animals
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Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Anaerobe
Ano de publicação:
2020
Tipo de documento:
Article