Derivatives of Deoxypodophyllotoxin Induce Apoptosis through Bcl-2/Bax Proteins Expression.
Anticancer Agents Med Chem
; 21(5): 611-620, 2021.
Article
em En
| MEDLINE
| ID: mdl-32748757
ABSTRACT
BACKGROUND:
Deoxypodophyllotoxin, isolated from the Traditional Chinese Medicine Anthriscus sylvestris, is well-known because of its significant anti-tumor activity with strong toxicity in vitro and in vivo.OBJECTIVE:
In this article, a series of deoxypodophyllotoxin derivatives were synthesized and their anti-tumor effectiveness was evaluated.METHODS:
The anti-tumor activity of deoxypodophyllotoxin derivatives was investigated by the MTT assay method. Apoptosis percentage was measured by flow cytometer analysis using Annexin-V-FITC.RESULTS:
The derivatives revealed obvious cytotoxicity in the MTT assay by decreasing the number of late cancer cells. The decrease of Bcl-2/Bax could be observed in MCF-7, HepG2, HT-29, and MG-63 using Annexin V-FITC. The ratio of Bcl-2/Bax in the administration group was decreased, which was determined by the ELISA kit.CONCLUSION:
The derivatives of deoxypodophyllotoxin could induce apoptosis in tumor cell lines by influencing Bcl-2/Bax.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Podofilotoxina
/
Medicamentos de Ervas Chinesas
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Proteína X Associada a bcl-2
/
Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Anticancer Agents Med Chem
Assunto da revista:
ANTINEOPLASICOS
/
QUIMICA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China