Human Bone Marrow Mesenchymal Stem Cell-derived Exosomes Attenuate IL-1ß-induced Annulus Fibrosus Cell Damage.
Am J Med Sci
; 360(6): 693-700, 2020 12.
Article
em En
| MEDLINE
| ID: mdl-32771218
ABSTRACT
BACKGROUND:
Intervertebral disc degeneration (IDD) has high morbidity and high disability. Exosomes as a favorable candidate for IDD treatment is a hot spot of current research.METHODS:
Exosomes were obtained from bone marrow mesenchymal stem cells (BM-MSCs) of patients with non-open femoral fractures. Then, annulus fibrosus (AF) cells were treated with IL-1ß, IL-1ß combined with exosomes or IL-1ß combined with exosomes and rapamycin. Flow cytometry and CCK-8 assay were performed to explore the apoptosis and cell proliferation. Quantitative real-time PCR and western blot were performed to detect the gene and protein expression.RESULTS:
Exosomes were obtained from BM-MSCs successfully. BM-MSC-derived exosomes suppressed IL-1ß-induced inflammation and apoptosis and promoted cell proliferation of AF cells. BM-MSC-derived exosomes inhibited autophagy of AF cells by activating PI3K/AKT/mTOR signaling pathway. The effect of BM-MSC-derived exosomes on inflammation and apoptosis of AF cells was rescued by rapamycin.CONCLUSIONS:
In conclusion, our study revealed that BM-MSC-derived exosomes inhibited IL-1ß-induced inflammation and apoptosis of AF cells by suppressing autophagy.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Apoptose
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Proliferação de Células
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Interleucina-1beta
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Exossomos
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Células-Tronco Mesenquimais
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Anel Fibroso
Idioma:
En
Revista:
Am J Med Sci
Ano de publicação:
2020
Tipo de documento:
Article