Nocardia macrotermitis sp. nov. and Nocardia aurantia sp. nov., isolated from the gut of the fungus-growing termite Macrotermes natalensis.
Int J Syst Evol Microbiol
; 70(10): 5226-5234, 2020 Oct.
Article
em En
| MEDLINE
| ID: mdl-32815801
ABSTRACT
The taxonomic positions of two novel aerobic, Gram-stain-positive Actinobacteria, designated RB20T and RB56T, were determined using a polyphasic approach. Both were isolated from the fungus-farming termite Macrotermes natalensis. Results of 16S rRNA gene sequence analysis revealed that both strains are members of the genus Nocardia with the closest phylogenetic neighbours Nocardia miyunensis JCM12860T (98.9â%) and Nocardia nova DSM44481T (98.5â%) for RB20T and Nocardia takedensis DSM 44801T (98.3â%), Nocardia pseudobrasiliensis DSM 44290T (98.3â%) and Nocardia rayongensis JCM 19832T (98.2â%) for RB56T. Digital DNA-DNA hybridization (DDH) between RB20T and N. miyunensis JCM12860T and N. nova DSM 44481T resulted in similarity values of 33.9 and 22.0â%, respectively. DDH between RB56T and N. takedensis DSM44801T and N. pseudobrasiliensis DSM44290T showed similarity values of 20.7 and 22.3â%, respectively. In addition, wet-lab DDH between RB56T and N. rayongensis JCM19832T resulted in 10.2â% (14.5â%) similarity. Both strains showed morphological and chemotaxonomic features typical for the genus Nocardia, such as the presence of meso-diaminopimelic acid (A2pm) within the cell wall, arabinose and galactose as major sugar components within whole cell-wall hydrolysates, the presence of mycolic acids and major phospholipids (diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol), and the predominant menaquinone MK-8 (H4, ω-cyclo). The main fatty acids for both strains were hexadecanoic acid (C16ââ0), 10-methyloctadecanoic acid (10-methyl C18ââ0) and cis-9-octadecenoic acid (C18ââ1 ω9c). We propose two novel species within the genus Nocardia Nocardia macrotermitis sp. nov. with the type strain RB20T (=VKM Ac-2841T=NRRL B65541T) and Nocardia aurantia sp. nov. with the type strain RB56T (=VKM Ac-2842T=NRRL B65542T).
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Isópteros
/
Nocardia
Limite:
Animals
País/Região como assunto:
Africa
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Alemanha