A sensitive biosensor for determination of pathogenic bacteria using aldehyde dehydrogenase signaling system.
Anal Bioanal Chem
; 412(28): 7955-7962, 2020 Nov.
Article
em En
| MEDLINE
| ID: mdl-32879993
ABSTRACT
Aldehyde dehydrogenase (ALDH) was first developed as an enzymatic signaling system of a biosensor for sensitive point-of-care detection of pathogenic bacteria. ALDH and specific aptamers to Salmonella typhimurium (S. typhimurium), as organic components, were embedded in organic-inorganic nanocomposites as a biosensor signal label, integrating the functions of signal amplification and target recognition. The biosensing mechanism is based on the fact that ALDH can catalyze rapid oxidation of acetaldehyde into acetic acid, resulting in pH change with portable pH meter readout. The altered pH exhibited a linear relationship with the logarithm of S. typhimurium from 102 to 108 CFU/mL and detection limit of 46 CFU/mL. Thus, the proposed biosensor has potential application in the diagnosis of pathogenic bacteria.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Salmonella typhimurium
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Transdução de Sinais
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Aldeído Desidrogenase
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Anal Bioanal Chem
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China