The Ago2-miRNA-co-IP Assay to Study TGF- ß1 Mediated Recruitment of miRNA to the RISC in CFBE Cells.

Mitash, Nilay; Donovan, Joshua E; Swiatecka-Urban, Agnieszka

Mitash, Nilay; Donovan, Joshua E; Swiatecka-Urban, Agnieszka.

Afiliação

Mitash N; Department of Pediatrics, UPMC Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine; Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, 200 Lothrop Street, University of Pittsburgh, Pittsburgh, PA, 15261; NIM109@pitt.edu.
Donovan JE; Department of Pediatrics, UPMC Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine; Department of Pediatric Nephrology, University of Virginia, Charlottesville, VA, 22908.
Swiatecka-Urban A; Department of Pediatrics, UPMC Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine; Department of Pediatric Nephrology, University of Virginia, Charlottesville, VA, 22908.

J Vis Exp ; (161)2020 07 31.

Article em En | MEDLINE | ID: mdl-32894261

Assuntos

Proteínas Argonautas/fisiologia; MicroRNAs/metabolismo; Complexo de Inativação Induzido por RNA/metabolismo; Fator de Crescimento Transformador beta1/farmacologia; Células Cultivadas; Humanos; Imunoprecipitação

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Complexo de Inativação Induzido por RNA / MicroRNAs / Fator de Crescimento Transformador beta1 / Proteínas Argonautas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Vis Exp Ano de publicação: 2020 Tipo de documento: Article

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