Diabetes and multidrug-resistance gene mutation: tuberculosis in Zunyi, Southwest China.
Ann Palliat Med
; 9(5): 3152-3161, 2020 Sep.
Article
em En
| MEDLINE
| ID: mdl-32921122
ABSTRACT
BACKGROUND:
The aim of this study was to clarify the characteristics of gene mutation related to multidrug-resistance (MDR) of tuberculosis (TB) and diabetes in Zunyi.METHODS:
A total 763 patients with TB were screened for TB-DNA, TB-RNA, and acid-fast staining (all were positive). They were divided into the tuberculosis (TB) group and the diabetes mellitus-tuberculosis (DM-TB) group. We compared and analyzed the MDR gene rpoB, KatG, and inhA characteristics of gene mutations in the two groups by polymerase chain reaction (PCR)-reverse dot hybridization, and collected relevant clinical data to explore its correlation with the occurrence of multidrug resistance.RESULTS:
Multidrug resistance occurred in 32 of the 525 patients in the TB group, and extensive drug resistance occurred in 15 of the 207 patients in the DM-TB group. In the DM-TB group, the mutation rates of ropBS531L and ropB531 (both 53.33%) were lower than those of the TB group (both 59.38%) in rifampicin resistance mutations. Most of the mutations were at the KatG315N site, conferring isoniazid resistance.CONCLUSIONS:
The mutation sites of multidrug-resistant patients in Zunyi are mainly ropB531 and ropBS531L mutations, which are prone to co-occurrence; patients with MDR-TB alone are prone to mutations at the KatG315N site, while patients with diabetes and MDR-TB are more likely to have inhA15M site mutations.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tuberculose
/
Diabetes Mellitus
/
Mycobacterium tuberculosis
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Ann Palliat Med
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China