Ex Vivo Culture for Preimplantation Mouse Embryo to Analyze Pluripotency.
Methods Mol Biol
; 2214: 1-10, 2021.
Article
em En
| MEDLINE
| ID: mdl-32944899
ABSTRACT
A couple of days after fertilization of a mouse oocyte by a sperm, two sequential cell differentiation events segregate pluripotent cells that can be identified by the presence of specific markers. Early mammalian embryos are relatively easy to recover as they are not yet implanted in the uterus matrix. Several decades of experimentation have enabled to find appropriate media to culture them, and therefore provide an excellent way to test different experimental setups such as the use of signaling inhibitors. We provide here a commonly used protocol to culture preimplantation embryos as well as a method to detect pluripotent cells in blastocysts.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Blastocisto
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Células-Tronco Pluripotentes
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Técnicas de Cultura Embrionária
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Camundongos
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
França