Applications and research advance of genome shuffling for industrial microbial strains improvement.
World J Microbiol Biotechnol
; 36(10): 158, 2020 Sep 24.
Article
em En
| MEDLINE
| ID: mdl-32968940
ABSTRACT
Genome shuffling, an efficient and practical strain improvement technology via recursive protoplasts fusion, can break through the limits of species even genus to accelerate the directed evolution of microbial strains, without requiring the comprehensively cognized genetic background and operable genetic system. Hence this technology has been widely used for many important strains to obtain the desirable industrial phenotypes. In this review, we introduce the procedure of genome shuffling, discuss the new aid strategies of genome shuffling, summarize the applications of genome shuffling for increasing metabolite yield, improving strain tolerance, enhancing substrate utilization, and put forward the outlook to the future development of this technology.
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Embaralhamento de DNA
Idioma:
En
Revista:
World J Microbiol Biotechnol
Ano de publicação:
2020
Tipo de documento:
Article