Iodine-131 labeled genistein as a potential radiotracer for breast cancer.
Heliyon
; 6(9): e04780, 2020 Sep.
Article
em En
| MEDLINE
| ID: mdl-33005774
ABSTRACT
OBJECTIVE:
Genistein is an isoflavone compound that has been proven to have anticancer activity and is capable of binding to estrogen ß receptors with Selective Estrogen Receptor Modulators (SERMs) properties, and has a strong affinity to inhibit the development of cancer cells. This study is to determine the optimum conditions of the reaction in the synthesis process of compounds labeled 131I-genestein which can be potential for application of breast cancer diagnosis.METHODS:
Synthesis of 131I-Genistein compound labeling using the Chloramine-T iodination method. This method uses several parameter optimizations, including pH conditions, the amount of chloramine-T oxidizer and sodium metabisulfite reducing agent. The radiochemical purity of the 131I-Genistein compound was determined using thin layer chromatography TLC-SG F254, and measured by SCA (Single Channel Analyzer). The radiochemical purity of labeled compounds must fulfill the requirements of the United States of Pharmacopeia.RESULTS:
Optimization of the synthesis conditions of the 131I-Genistein compound was obtained at pH 8, the amount of chloramine-T 0.225 mg, and the amount of Na-Metabisulfite 0.342 mg, with 30 min reaction time. This optimum condition produces radiochemical purity of 95.02 ± 0.76%.CONCLUSION:
Products labeled 131I-Genistein meet radiochemical purity requirements according to USP requirements. The labeled compound is expected to be able to be used to detect breast cancer through a binding mechanism with estrogen receptors ß.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
Heliyon
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Indonésia