Void-free 3D Bioprinting for In-situ Endothelialization and Microfluidic Perfusion.
Adv Funct Mater
; 30(1)2020 Jan 03.
Article
em En
| MEDLINE
| ID: mdl-33071714
ABSTRACT
Two major challenges of 3D bioprinting are the retention of structural fidelity and efficient endothelialization for tissue vascularization. We address both of these issues by introducing a versatile 3D bioprinting strategy, in which a templating bioink is deposited layer-by-layer alongside a matrix bioink to establish void-free multimaterial structures. After crosslinking the matrix phase, the templating phase is sacrificed to create a well-defined 3D network of interconnected tubular channels. This void-free 3D printing (VF-3DP) approach circumvents the traditional concerns of structural collapse, deformation and oxygen inhibition, moreover, it can be readily used to print materials that are widely considered "unprintable". By pre-loading endothelial cells into the templating bioink, the inner surface of the channels can be efficiently cellularized with a confluent endothelial layer. This in-situ endothelialization method can be used to produce endothelium with a far greater uniformity than can be achieved using the conventional post-seeding approach. This VF-3DP approach can also be extended beyond tissue fabrication and towards customized hydrogel-based microfluidics and self-supported perfusable hydrogel constructs.
Texto completo:
1
Base de dados:
MEDLINE
Idioma:
En
Revista:
Adv Funct Mater
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Reino Unido