An impedimetric assay for the identification of abnormal mitochondrial dynamics in living cells.
Electrophoresis
; 42(1-2): 163-170, 2021 01.
Article
em En
| MEDLINE
| ID: mdl-33169407
ABSTRACT
Mitochondrial dynamics (fission and fusion) plays an important role in cell functions. Disruption in mitochondrial dynamics has been associated with diseases such as neurobiological disorders and cardiovascular diseases. Analysis of mitochondrial fission/fusion has been mostly achieved through direct visualization of the fission/fusion events in live-cell imaging of fluorescently labeled mitochondria. In this study, we demonstrated a label-free, non-invasive Electrical Impedance Spectroscopy (EIS) approach to analyze mitochondrial dynamics in a genetically modified human neuroblastoma SH-SY5Y cell line with no huntingtin protein expression. Huntingtin protein has been shown to regulate mitochondria dynamics. We performed EIS studies on normal SH-SY5Y cells and two independent clones of huntingtin-null cells. The impedance data was used to determine the suspension conductivity and further cytoplasmic conductivity and relate to the abnormal mitochondrial dynamics. For instance, the cytoplasm conductivity value was increased by 11% from huntingtin-null cells to normal cells. Results of this study demonstrated that EIS is sensitive to characterize the abnormal mitochondrial dynamics that can be difficult to quantify by the conventional microscopic method.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral
/
Técnicas Citológicas
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Dinâmica Mitocondrial
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos