Protective role of fermented mulberry leave extract in LPSinduced inflammation and autophagy of RAW264.7 macrophage cells.
Mol Med Rep
; 22(6): 4685-4695, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-33174019
ABSTRACT
Mulberry leaves have antioxidant activity and antiinflammatory effects in several types of cells. However, the efficacy of mulberry leaves fermented with Cordyceps militaris remains unknown. Therefore, the present study aimed to investigate whether the ethanol extracts of mulberry leaves fermented with C. militaris (EMfC) can prevent lipopolysaccharide (LPS)induced inflammation and autophagy in macrophages. To achieve this, RAW264.7 cells pretreated with three different dose of EMfCs were subsequently stimulated with LPS, and examined for alterations in the regulatory factors of inflammatory responses and key parameters of the autophagy signaling pathway. EMfC treatment inhibited the generation of reactive oxidative species; however, significant activity was observed for 2,2diphenyl1picrylhydrazyl (DPPH) radical scavenging (IC50=579.6703 mg/ml). Most regulatory factors in inflammatory responses were significantly inhibited following treatment with EMfC, without any significant cellular toxicity. EMfCtreated groups exhibited marked suppression of nitrogen oxide (NO) levels, mRNA expression levels of iNOS/COX2, levels of all inflammatory cytokines (TNFα, IL1ß and IL6) and phosphorylation of MAPK members, as well as recovery of cell cycle progression. Furthermore, similar effects were observed in the LPSinduced autophagy signaling pathway of RAW264.7 cells. The expression levels of microtubuleassociated protein 1A/1Blight chain 3 (LC3) and Beclin exhibited a dosedependent decrease in the EMfC+LPStreated groups compared with in the Vehicle+LPStreated group, whereas the phosphorylation of PI3K and mTOR were enhanced in a dosedependent manner in the same groups. Overall, the results of the present study provide evidence that exposure to EMfC protects against LPSinduced inflammation and autophagy in RAW264.7 cells. These results indicated that EMfC is a potential candidate for treatment of inflammatory diseases.
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Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
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Morus
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Inflamação
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2020
Tipo de documento:
Article