MicroRNA-141-3p promoted the progression of nasopharyngeal carcinoma through targeting DLC1.
Eur Rev Med Pharmacol Sci
; 24(21): 11105-11113, 2020 11.
Article
em En
| MEDLINE
| ID: mdl-33215427
ABSTRACT
OBJECTIVE:
Previous studies have shown that the function of miR-141 has tissue specificity. However, the role of miR-141-3p has not been reported in nasopharyngeal carcinoma (NPC). Therefore, this study explored the function of miR-141-3p in NPC. PATIENTS ANDMETHODS:
MiR-141-3p expression in NPC tissues was examined via quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) assay. Cell Counting Kit-8 (CCK-8) and transwell assays were used to explore the function of miR-141-3p. The relationship between miR-141-3p and DLC1 was verified by Dual-Luciferase assay. Protein expression was observed by immunocytochemical assay and Western blot analysis.RESULTS:
Upregulation of miR-141-3p associated with poor prognosis was detected in NPC patients. Moreover, overexpression of miR-141-3p promoted cell proliferation, migration, and invasion in NPC cells. It was also found that miR-141-3p promoted EMT and activated the mTOR signaling pathway in NPC. Furthermore, DLC1 was indicated as a direct target of miR-141-3p and miR-141-3p negatively correlated with DLC1 expression in NPC. In particular, upregulation of DLC1 could impair the promoted effect of miR-141-3p in NPC.CONCLUSIONS:
MiR-141-3p promotes the progression of NPC by targeting DLC1 and activating the mTOR pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Nasofaríngeas
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Proteínas Ativadoras de GTPase
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Proteínas Supressoras de Tumor
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MicroRNAs
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Carcinoma Nasofaríngeo
Limite:
Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Assunto da revista:
FARMACOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China