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Tribbles-1 Expression and Its Function to Control Inflammatory Cytokines, Including Interleukin-8 Levels are Regulated by miRNAs in Macrophages and Prostate Cancer Cells.
Niespolo, Chiara; Johnston, Jessica M; Deshmukh, Sumeet R; Satam, Swapna; Shologu, Ziyanda; Villacanas, Oscar; Sudbery, Ian M; Wilson, Heather L; Kiss-Toth, Endre.
Afiliação
  • Niespolo C; Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield, Sheffield, United Kingdom.
  • Johnston JM; Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield, Sheffield, United Kingdom.
  • Deshmukh SR; Department of Molecular Biology and Biotechnology, Sheffield Institute for Nucleic Acids, University of Sheffield, Sheffield, United Kingdom.
  • Satam S; Institute for Diabetes and Cancer IDC, Helmholtz Center, Munich, Germany.
  • Shologu Z; Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal.
  • Villacanas O; Intelligent Pharma, Barcelona, Spain.
  • Sudbery IM; Department of Molecular Biology and Biotechnology, Sheffield Institute for Nucleic Acids, University of Sheffield, Sheffield, United Kingdom.
  • Wilson HL; Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield, Sheffield, United Kingdom.
  • Kiss-Toth E; Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield, Sheffield, United Kingdom.
Front Immunol ; 11: 574046, 2020.
Article em En | MEDLINE | ID: mdl-33329538
ABSTRACT
The pseudokinase TRIB1 controls cell function in a range of contexts, by regulating MAP kinase activation and mediating protein degradation via the COP1 ubiquitin ligase. TRIB1 regulates polarization of macrophages and dysregulated Trib1 expression in murine models has been shown to alter atherosclerosis burden and adipose homeostasis. Recently, TRIB1 has also been implicated in the pathogenesis of prostate cancer, where it is often overexpressed, even in the absence of genetic amplification. Well described TRIB1 effectors include MAP kinases and C/EBP transcription factors, both in immune cells and in carcinogenesis. However, the mechanisms that regulate TRIB1 itself remain elusive. Here, we show that the long and conserved 3'untranslated region (3'UTR) of TRIB1 is targeted by miRNAs in macrophage and prostate cancer models. By using a systematic in silico analysis, we identified multiple "high confidence" miRNAs potentially binding to the 3'UTR of TRIB1 and report that miR-101-3p and miR-132-3p are direct regulators of TRIB1 expression and function. Binding of miR-101-3p and miR-132-3p to the 3'UTR of TRIB1 mRNA leads to an increased transcription and secretion of interleukin-8. Our data demonstrate that modulation of TRIB1 by miRNAs alters the inflammatory profile of both human macrophages and prostate cancer cells.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Citocinas / Proteínas Serina-Treonina Quinases / MicroRNAs / Peptídeos e Proteínas de Sinalização Intracelular / Macrófagos Tipo de estudo: Prognostic_studies Limite: Animals / Humans / Male Idioma: En Revista: Front Immunol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Reino Unido

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Citocinas / Proteínas Serina-Treonina Quinases / MicroRNAs / Peptídeos e Proteínas de Sinalização Intracelular / Macrófagos Tipo de estudo: Prognostic_studies Limite: Animals / Humans / Male Idioma: En Revista: Front Immunol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Reino Unido