Model of Walnut Allergy in CC027/GeniUnc Mice Recapitulates Key Features of Human Disease.
Yale J Biol Med
; 93(5): 669-673, 2020 12.
Article
em En
| MEDLINE
| ID: mdl-33380927
ABSTRACT
Tree nut allergies affect 1% of the United States population, are often severe in nature and rarely outgrown. Despite the severity and prevalence, there are no FDA-approved treatments for tree nut allergy. Development of a therapeutic would be expedited by having a mouse model that mimics the human disease. We utilized the CC027/GeniUnc mouse strain, which was previously identified as an orally reactive model of peanut allergy, to develop a model of walnut allergy. Mice were sensitized with walnut and cholera toxin for 4 weeks and subsequently challenged by oral gavage. Blood samples were collected to measure serum IgE. Walnut-sensitized mice produced high levels of walnut-IgE and were cross-sensitized to pecan. Oral challenges with walnut resulted in severe anaphylaxis and accompanying allergic symptoms. Importantly, pecan challenges also led to severe allergic reactions, indicating cross-reactivity to pecan. Overall, this novel mouse model reproduces key characteristics of human walnut allergy, which provides a platform to develop novel therapies and better understand sensitization mechanisms.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hipersensibilidade a Noz
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Hipersensibilidade a Amendoim
/
Juglans
Tipo de estudo:
Prevalence_studies
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Prognostic_studies
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Risk_factors_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
Yale J Biol Med
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Nova Caledônia