circHIPK3 promotes proliferation and migration and invasion via regulation of miR637/HDAC4 signaling in osteosarcoma cells.
Oncol Rep
; 45(1): 169-179, 2021 01.
Article
em En
| MEDLINE
| ID: mdl-33416147
ABSTRACT
Accumulating evidence has indicated that circular RNAs (circRNAs) serve crucial roles in the progression of a diverse range of different types of cancer, including osteosarcoma (OS). The present study determined the expression pattern and function of circRNA homeodomain interacting protein kinase 3 (circHIPK3), a novel circular RNA, in OS. It was revealed that circHIPK3 expression was upregulated in OS tissue samples and OS cell lines. A localization assay revealed that circHIPK3 was primarily located in the cytoplasm. Using lossoffunction proliferation and Transwell assays, the present study revealed that circHIPK3knockdown suppressed OS cell proliferation, migration and invasion. Furthermore, the present study screened potential microRNAs that may interact with circHIPK3. It was revealed that microRNA637 (miR637) expression was downregulated in OS according to a Gene Expression Omnibus data analysis. In addition, the present study demonstrated that miR637 expression was downregulated in OS cell lines. A fluorescence in situ hybridization assay revealed that both miR637 and circHIPK3 were located in the cytoplasm. An indepth mechanism investigation demonstrated that circHIPK3 expression was inversely correlated with miR637 expression, and that circHIPK3 was a target of miR637. In addition, it was revealed that histone deacetylase 4 (HDAC4) was another downstream target gene of miR637, as demonstrated using a luciferase assay. It was revealed that miR637 suppressed OS cell proliferation, migration and invasion via targeting of HDAC4. Finally, the present study demonstrated that circHIPK3 sponged miR637 to promote HDAC4 expression and OS cell proliferation, migration and invasion. In conclusion, the present study uncovered the role of the circHIPK3/miR637/HDAC4 axis in OS cell proliferation, migration and invasion. It was demonstrated that circHIPK3 promoted OS cell proliferation, migration and invasion by modulating miR637/HDAC4 signaling.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
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Neoplasias Ósseas
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Osteossarcoma
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MicroRNAs
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RNA Circular
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Histona Desacetilases
Tipo de estudo:
Observational_studies
Limite:
Adolescent
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Adult
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Child
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Oncol Rep
Assunto da revista:
NEOPLASIAS
Ano de publicação:
2021
Tipo de documento:
Article