A lysosomal targeted NIR photosensitizer for photodynamic therapy and two-photon fluorescence imaging.
J Mater Chem B
; 9(4): 1009-1017, 2021 01 28.
Article
em En
| MEDLINE
| ID: mdl-33427275
ABSTRACT
The design and synthesis of near-infrared (NIR) emissive fluorophores for the imaging of organelles and photodynamic therapy have received enormous attention. Hence, the development of NIR emissive fluorophores for high-fidelity lysosome targeting, two-photon fluorescence imaging, and the inducing of photo-triggered cancer-cell apoptosis is highly desirable. In this study, a novel lysosome-targeting two-photon fluorescent photosensitizer (TTRh-CN) is prepared and comprehensively investigated. TTRh-CN demonstrates near-infrared (NIR) emission, good biocompatibility, and superior photostability, and it can act as a two-photon fluorescent agent for the clear visualization of living cells and the vascular system within tissue, with deep-tissue penetration abilities. Furthermore, TTRh-CN can efficiently produce ROS in conjunction with lysosomes in situ upon light irradiation, which can damage lysosomes, up-regulate LC3 and Beclin1, increase BAX release, and induce cell apoptosis. The efficacy of TTRh-CN as a photosensitizer is explored in vivo. All these results confirm that TTRh-CN can serve as a potential platform for the two-photon fluorescence imaging of cells/tissue and for organelle-specific photodynamic therapy.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fotoquimioterapia
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Materiais Biocompatíveis
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Fármacos Fotossensibilizantes
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Fótons
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Imagem Óptica
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Antineoplásicos
Limite:
Animals
Idioma:
En
Revista:
J Mater Chem B
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China