Dual-probe fluorescent biosensor based on T7 exonuclease-assisted target recycling amplification for simultaneous sensitive detection of microRNA-21 and microRNA-155.

Zheng, Yanjie; Chen, Jinyuan; Li, You; Xu, Yichun; Chen, Li; Chen, Wei; Liu, Ailin; Lin, Xinhua; Weng, Shaohuang

Zheng, Yanjie; Chen, Jinyuan; Li, You; Xu, Yichun; Chen, Li; Chen, Wei; Liu, Ailin; Lin, Xinhua; Weng, Shaohuang.

Afiliação

Zheng Y; Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, China.
Chen J; The Central lab, the First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, China.
Li Y; Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, China.
Xu Y; Department of Pharmacy, Fuzhou Pulmonary Hospital of Fujian, Fuzhou, 350008, Fujian, China.
Chen L; Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, China.
Chen W; Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, China.
Liu A; Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, China.
Lin X; Nano Biomedical Technology Research Center, Fujian Medical University, Fuzhou, 350122, Fujian, China.
Weng S; Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, China.

Anal Bioanal Chem ; 413(6): 1605-1614, 2021 Mar.

Article em En | MEDLINE | ID: mdl-33515273

Assuntos

Técnicas Biossensoriais; Exodesoxirribonucleases/metabolismo; Neoplasias Pulmonares/sangue; MicroRNAs/análise; Sondas de DNA/química; Polarização de Fluorescência; Grafite/química; Humanos; Limite de Detecção; Neoplasias Pulmonares/genética; Neoplasias Pulmonares/metabolismo; Técnicas de Amplificação de Ácido Nucleico/métodos; Plasma/metabolismo; Reprodutibilidade dos Testes; Espectrometria de Fluorescência/métodos

Palavras-chave

Drug resistance warning; Dual-probe fluorescent biosensor; Simultaneous detection; microRNA-155; microRNA-21

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / MicroRNAs / Exodesoxirribonucleases / Neoplasias Pulmonares Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Anal Bioanal Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

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